This laboratory course applies concepts learned in Cell and Molecular Biology lecture to a molecular biology research project, with an aim to introduce students to in vitro techniques utilized in a modern biological research lab. While it is not possible to cover all the methods and technologies in a single semester, the fundamental skills taught in this course will provide a basis for understanding many more complex approaches. Topics covered include ribose nucleic acic (RNA) isolation, agarose gel electrophoresis, polymerase chain reaction, restriction enzyme digestion, deoxyribose nucleic acid (DNA) cloning, bacterial transformation, plasmid deoxyribose nucleic acid (DNA) isolation, mammalian cell culture, deoxyribose nucleic acid (DNA) transfection, fluorescence microscopy, and sub-cellular fractionation.
